Dunphy W G, Brizuela L, Beach D, Newport J
Department of Biology, University of California, San Diego, La Jolla 92093.
Cell. 1988 Jul 29;54(3):423-31. doi: 10.1016/0092-8674(88)90205-x.
In Xenopus, a cytoplasmic agent known as MPF induces entry into mitosis. In fission yeast, genetic studies have shown that the cdc2 kinase regulates mitotic initiation. The 13 kd product of the suc1 gene interacts with the cdc2 kinase in yeast cells. We show that the yeast suc1 gene product (p13) is a potent inhibitor of MPF in cell-free extracts from Xenopus eggs. p13 appears to exert its antagonistic effect by binding directly to MPF. MPF activity is quantitatively depleted by chromatography on a p13 affinity column. Concomitantly, the Xenopus counterpart of the yeast cdc2 protein is adsorbed to the column. A 42 kd protein also binds specifically to the p13 affinity matrix. These findings suggest that the Xenopus cdc2 protein and the 42 kd protein are components of MPF.
在非洲爪蟾中，一种名为MPF的细胞质因子可诱导细胞进入有丝分裂。在裂殖酵母中，遗传学研究表明cdc2激酶调节有丝分裂的起始。suc1基因的13kd产物在酵母细胞中与cdc2激酶相互作用。我们发现酵母suc1基因产物（p13）是非洲爪蟾卵无细胞提取物中MPF的有效抑制剂。p13似乎通过直接结合MPF发挥其拮抗作用。通过在p13亲和柱上进行层析，MPF活性被定量去除。同时，酵母cdc2蛋白的非洲爪蟾对应物被吸附到柱上。一种42kd的蛋白也特异性地结合到p13亲和基质上。这些发现表明非洲爪蟾cdc2蛋白和42kd蛋白是MPF的组成成分。
