Shimodaira S, Ishida F, Kobayashi H, Mahbub B, Kawa-Ha K, Kitano K
Second Department of Internal Medicine, Shinshu University School of Medicine, Matsumoto, Japan.
Br J Haematol. 1995 Jul;90(3):578-84. doi: 10.1111/j.1365-2141.1995.tb05587.x.
The clonal proliferation of large granular lymphocytes can be detected in patients with T-cell-lineage granular lymphocyte-proliferative disorders (T-GLPD) by Southern blotting T-cell receptor genes. However, this cannot be applied to patients with natural killer-cell-lineage GLPD (NK-GLPD) as it lacks a clonal marker. We therefore investigated the use of two other diagnostic techniques in evaluating clonal proliferation in Japanese patients with NK-GLPD (n = 4) and T-GLPD (n = 3) by chromosomal analysis of peripheral blood mononuclear cells (PBMC) stimulated with either interleukin-2 or phytohaemagglutinin, and Epstein-Barr viral (EBV) genomic DNA analysis. Chromosomal analysis revealed abnormal karyotypes in the PBMC of three of four patients with NK-GLPD, whereas EBV analysis showed a monoclonal terminal configuration in the PBMC in the fourth patient. Southern blots revealed rearrangements of the TCR genes in all three patients with T-GLPD but in none of those with NK-GLPD. It is suggested that these methods may be useful in detecting the abnormal proliferation of large granular lymphocytes in NK-GLPD.
通过Southern印迹法检测T细胞系颗粒淋巴细胞增殖性疾病（T-GLPD）患者的大颗粒淋巴细胞克隆增殖情况时，可检测T细胞受体基因。然而，这一方法不适用于自然杀伤细胞系GLPD（NK-GLPD）患者，因为其缺乏克隆标记物。因此，我们通过对用白细胞介素-2或植物血凝素刺激的外周血单个核细胞（PBMC）进行染色体分析以及爱泼斯坦-巴尔病毒（EBV）基因组DNA分析，研究了另外两种诊断技术在评估日本NK-GLPD患者（n = 4）和T-GLPD患者（n = 3）克隆增殖中的应用。染色体分析显示，4例NK-GLPD患者中有3例的PBMC核型异常，而EBV分析显示第4例患者的PBMC中有单克隆末端构型。Southern印迹显示，所有3例T-GLPD患者的TCR基因均有重排，而NK-GLPD患者均无重排。提示这些方法可能有助于检测NK-GLPD中大颗粒淋巴细胞的异常增殖。
