通过准弹性中子散射研究超氧化物歧化酶中氢原子的动力学
Dynamics of hydrogen atoms in superoxide dismutase by quasielastic neutron scattering.
作者信息
Andreani C, Filabozzi A, Menzinger F, Desideri A, Deriu A, Di Cola D
机构信息
Dipartimento di Fisica, Università di Roma Tor Vergata, Italy.
出版信息
Biophys J. 1995 Jun;68(6):2519-23. doi: 10.1016/S0006-3495(95)80434-0.
Abstract
The low energy dynamic of the enzyme Cu,Zn superoxide dismutase have been investigated by means of quasielastic neutron scattering in the temperature range 4-320 K. Below 200 K the scattering is purely elastic, while above this temperature a pronounced decrease in the elastic intensity is observed, together with the onset of a small quasielastic component. This behavior is similar to that previously observed in other more flexible globular proteins, and can be attributed to transitions between slightly different conformational substates of the protein tertiary structure. The presence of only a small quasielastic component, whose intensity is < or = 25% of the total spectrum, is related to the high structural rigidity of this protein.
摘要
通过在4 - 320 K温度范围内的准弹性中子散射研究了铜锌超氧化物歧化酶的低能动力学。在200 K以下，散射是纯弹性的，而高于此温度时，观察到弹性强度明显下降，同时出现一个小的准弹性分量。这种行为与先前在其他更灵活的球状蛋白质中观察到的行为相似，并且可归因于蛋白质三级结构的略有不同构象亚态之间的转变。仅存在一个小的准弹性分量，其强度小于或等于总光谱的25%，这与该蛋白质的高结构刚性有关。
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本文引用的文献
1
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Phys Rev Lett. 1990 Aug 20;65(8):1080-1083. doi: 10.1103/PhysRevLett.65.1080.
2
Observation of acoustic umklapp-phonons in water-stabilized DNA by neutron scattering.通过中子散射观测水稳定DNA中的声学倒逆声子
Phys Rev Lett. 1987 Oct 12;59(15):1780-1783. doi: 10.1103/PhysRevLett.59.1780.
3
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Biochem Biophys Res Commun. 1993 Aug 16;194(3):1008-11. doi: 10.1006/bbrc.1993.1921.
4
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Proteins. 1993 Jun;16(2):141-54. doi: 10.1002/prot.340160203.
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6
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7
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10
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