大鼠实验性变应性神经炎中T淋巴细胞亚群的双色分析

The two-color analysis of the T-lymphocyte subsets in experimental allergic neuritis in rats.

作者信息

Takai H, Araga S, Funamoto K, Nakayasu H, Takahashi K

机构信息

Division of Neurology, Faculty of Medicine, Tottori University, Yonago, Japan.

出版信息

Intern Med. 1995 May;34(5):347-51. doi: 10.2169/internalmedicine.34.347.

DOI:10.2169/internalmedicine.34.347

PMID:7647400

Abstract

We examined the T-cell subsets in the peripheral blood, spleen and lymph nodes during the course of experimental allergic neuritis (EAN) by using two-color analysis. In the acute phase, the percentages of CD4+ major histocompatibility complex (MHC)-II+ cells and CD8+ MHC-II+ cells in the lymph nodes of EAN rats were significantly higher than in the control rats. In the recovery phase, the percentage of CD4+ CD45RC+ cells and CD8+ CD45RC+ cells in the lymph nodes in EAN rats were significantly higher than in the control rats. However, significant changes of the T-cell subsets were not detected in either the spleen or the peripheral blood during the course of EAN. These results suggest that CD4+ MHC-II+ cells, CD8+ MHC-II+ cells, CD4+ CD45RC+ cells and CD8+ CD45RC+ cells may play a role in the course of EAN. The relationship between these double staining cells and EAN is discussed.

摘要

我们运用双色分析方法，对实验性变应性神经炎（EAN）病程中大鼠外周血、脾脏及淋巴结中的T细胞亚群进行了检测。在急性期，EAN大鼠淋巴结中CD4⁺主要组织相容性复合体（MHC）-II⁺细胞和CD8⁺ MHC-II⁺细胞的百分比显著高于对照大鼠。在恢复期，EAN大鼠淋巴结中CD4⁺ CD45RC⁺细胞和CD8⁺ CD45RC⁺细胞的百分比显著高于对照大鼠。然而，在EAN病程中，无论是脾脏还是外周血中的T细胞亚群均未检测到显著变化。这些结果提示，CD4⁺ MHC-II⁺细胞、CD8⁺ MHC-II⁺细胞、CD4⁺ CD45RC⁺细胞和CD8⁺ CD45RC⁺细胞可能在EAN病程中发挥作用。本文讨论了这些双重染色细胞与EAN之间的关系。