Louis E, Franchimont D, Lamproye A, van Kemseke C, Schaaf N, Mahieu P, Belaiche J
Department of Gastroenterology, CHU of Liège, Belgium.
Int Arch Allergy Immunol. 1995 Sep;108(1):19-23. doi: 10.1159/000237112.
The aim of our study was to determine the effect of a rectocolonic preimmunization with ovalbumin on the systemic immune response induced by a subsequent subcutaneous injection of this antigen in Balb/c mice. One rectocolonic, but not intragastric, administration of 25 mg of ovalbumin induced a detectable increase in serum anti-ovalbumin antibody level. The level reached was however much lower than after subcutaneous injection. Both intragastric and rectocolonic immunization with ovalbumin induced specific systemic cellular tolerance. However, after rectocolonic, but not intragastric, preimmunization there was no systemic humoral tolerance to this antigen. These differences in systemic immune responses after rectocolonic or intragastric administration of ovalbumin could be due to different stimulation of the systemic immune system or to differences between the colonic and small bowel mucosal immune system, which remain to be elucidated.
我们研究的目的是确定用卵清蛋白对结肠直肠进行预免疫，对随后在Balb/c小鼠皮下注射该抗原所诱导的全身免疫反应的影响。给予25 mg卵清蛋白进行一次结肠直肠给药（而非胃内给药）可使血清抗卵清蛋白抗体水平出现可检测到的升高。然而，所达到的水平远低于皮下注射后的水平。用卵清蛋白进行胃内和结肠直肠免疫均可诱导特异性全身细胞耐受性。但是，在进行结肠直肠预免疫（而非胃内预免疫）后，对该抗原不存在全身体液耐受性。结肠直肠或胃内给予卵清蛋白后全身免疫反应的这些差异，可能是由于对全身免疫系统的刺激不同，或者是由于结肠和小肠黏膜免疫系统之间的差异，这些仍有待阐明。
