Nagamine T, Saito S, Kaneko M, Sekiguchi T, Sugimoto H, Takehara K, Takagi H
First Department of Internal Medicine, Gunma University School of Medicine, Maebashi, Japan.
J Gastroenterol. 1995 Jun;30(3):351-5. doi: 10.1007/BF02347511.
The effects of biotin on ammonia concentration in blood and brain were evaluated in hyperammonemic rats and mice. Rats were injected with 5 mmol/kg BW of ammonium acetate, and mice were injected with 10 mmol/kg BW. Increases in blood ammonia levels in rats 15-30 min after ammonia loading were prevented by treatment with 0.2 ml/100 g BW of biotin or 0.04 ml/100 g BW of arginine-glutamate with statistical significance. Blood ammonia levels after ammonia loading were lower, although not significantly, in the arginine glutamate-treated rats than in the biotin-treated animals. In mice also, increases in blood and brain ammonia levels after ammonia loading were prevented by the administration of biotin. The decrease in brain glutamate and aspartate after ammonia loading was lower and the brain glutamine level was higher in biotin-treated mice than in the controls. These findings indicate the protective effect of biotin against ammonia intoxication.
在高氨血症大鼠和小鼠中评估了生物素对血液和大脑中氨浓度的影响。给大鼠注射5 mmol/kg体重的醋酸铵，给小鼠注射10 mmol/kg体重。用0.2 ml/100 g体重的生物素或0.04 ml/100 g体重的精氨酸 - 谷氨酸处理可预防氨负荷后15 - 30分钟大鼠血氨水平的升高，具有统计学意义。精氨酸谷氨酸处理的大鼠氨负荷后的血氨水平低于生物素处理的动物，尽管差异不显著。在小鼠中，生物素的给药也可预防氨负荷后血液和大脑氨水平的升高。与对照组相比，生物素处理的小鼠氨负荷后大脑谷氨酸和天冬氨酸的降低幅度较小，大脑谷氨酰胺水平较高。这些发现表明生物素对氨中毒具有保护作用。
