Oh C H, Kim J H, Kim K R, Mabry T J
Department of Food and Biotechnology, Yonsei University, Seoul, South Korea.
J Chromatogr A. 1995 Jul 28;708(1):131-41. doi: 10.1016/0021-9673(95)00381-v.
Water extraction with subsequent picric acid treatment and solvent washing (ethyl acetate and diethyl ether) was employed for the rapid isolation of free amino acids from non-aqueous food samples. The isolated amino acids were subjected to N(O,S)-isobutyloxycarbonylation followed by solid-phase extraction and tert.-butyldimethylsilylation for direct analysis by gas chromatography and gas chromatography-mass spectrometry. When the present method was applied to nineteen food samples (common beans, seeds, and nuts), seventeen protein amino acids and fifteen non-protein amino acids were simultaneously screened. Eleven non-protein amino acids including gamma-aminobutyric acid, pipecolic acid, pyroglutamic acid, alpha-aminobutyric acid, alpha-aminoadipic acid, and beta-alanine were tentatively identified, and four compounds assumed to be non-protein amino acids remained unidentified.
采用水提取法，随后进行苦味酸处理和溶剂洗涤（乙酸乙酯和乙醚），用于从非水性食品样品中快速分离游离氨基酸。将分离出的氨基酸进行N（O，S）-异丁氧基羰基化，然后进行固相萃取和叔丁基二甲基硅烷化，以通过气相色谱和气相色谱-质谱联用进行直接分析。当将本方法应用于19种食品样品（普通豆类、种子和坚果）时，同时筛选出了17种蛋白质氨基酸和15种非蛋白质氨基酸。初步鉴定出11种非蛋白质氨基酸，包括γ-氨基丁酸、哌啶酸、焦谷氨酸、α-氨基丁酸、α-氨基己二酸和β-丙氨酸，还有4种被认为是非蛋白质氨基酸的化合物未被鉴定出来。
