Oh C H, Brownson D M, Mabry T J
Department of Botany, University of Texas, Austin 78713-7640.
Planta Med. 1995 Feb;61(1):66-70. doi: 10.1055/s-2006-958002.
The non-protein amino acid, beta-N-methyl-amino-L-alanine (L-BMAA) from Cycas circinalis seeds, has been implicated as a causative agent of amyotrophic lateral sclerosis-Parkinsonism dementia complex (ALS-PDC), a disease known from Guam and other areas in the western Pacific. We analyzed C. circinalis seeds for additional free non-protein amino acids by a recently developed GC-MS method. The samples were prepared by water extraction of seed flour. The amino acids present in the extract were derivatized by N(O,S)-isobutyloxycarbonylation of the amine functional groups and then tert-butyldimethylsilylation of the carboxyl functional groups. Peaks for a total of seventeen derivatives of non-protein amino acids were detected by GC-MS. In addition to L-BMAA, four other non-protein amino acids were identified as beta-alanine, gamma-amino-butyric acid, pyroglutamic acid, and alpha-aminoadipic acid.
来自卷圈苏铁种子的非蛋白质氨基酸β-N-甲基-L-丙氨酸（L-BMAA），被认为是肌萎缩侧索硬化-帕金森病痴呆综合征（ALS-PDC）的致病因子，这种疾病在关岛和西太平洋其他地区为人所知。我们通过最近开发的气相色谱-质谱联用（GC-MS）方法分析了卷圈苏铁种子中其他游离的非蛋白质氨基酸。样品通过种子粉的水提取制备。提取物中存在的氨基酸通过胺官能团的N（O，S）-异丁氧基羰基化，然后羧基官能团的叔丁基二甲基硅烷基化进行衍生化。通过GC-MS检测到总共十七种非蛋白质氨基酸衍生物的峰。除了L-BMAA之外，还鉴定出其他四种非蛋白质氨基酸，即β-丙氨酸、γ-氨基丁酸、焦谷氨酸和α-氨基己二酸。
