Hirokawa M, Shimizu M, Manabe T, Kuroda M, Mizoguchi Y
Department of Pathology, Kawasaki Medical School, Kurashiki, Japan.
Pathol Int. 1995 May;45(5):399-401. doi: 10.1111/j.1440-1827.1995.tb03475.x.
The monoclonal antibody Ki-67 reacts with a human nuclear cell proliferation-associated antigen that is expressed in all active parts of the cell cycle and is well established as a marker of cell proliferation. However, the Ki-67 method requires fresh frozen material. Recently, MIB1 has been reported to give an immunohistochemical staining pattern identical to Ki-67 on paraffin-embedded tissue sections, frozen sections and cytological samples. This proliferation-associated antigen is apparently localized in the nucleus. Recently, we performed immunohistochemical staining using the monoclonal MIB1 antibody upon a variety of tumors and non-neoplastic conditions of the thyroid. Tumor cells of hyalinizing trabecular adenoma revealed an intense cytoplasmic immunopositivity for MIB1. In contrast, cytoplasmic immunostaining for MIB1 was negative in all other thyroid tumors and non-neoplastic lesions. Because of this unusual staining pattern, we repeated the staining of all cases and found the results to be reproducible. Therefore, we believe that positive cytoplasmic immunostaining for MIB1 is a characteristic finding of hyalinizing trabecular adenoma and is useful in differentiating it from other thyroid tumors.
单克隆抗体Ki-67与一种人类核细胞增殖相关抗原发生反应，该抗原在细胞周期的所有活跃阶段均有表达，并且已被充分确立为细胞增殖的标志物。然而，Ki-67检测方法需要新鲜冷冻材料。最近，有报道称MIB1在石蜡包埋组织切片、冰冻切片和细胞学样本上的免疫组化染色模式与Ki-67相同。这种增殖相关抗原显然定位于细胞核中。最近，我们使用单克隆MIB1抗体对多种甲状腺肿瘤和非肿瘤性病变进行了免疫组化染色。透明变梁状腺瘤的肿瘤细胞显示出对MIB1强烈的细胞质免疫阳性。相比之下，在所有其他甲状腺肿瘤和非肿瘤性病变中，MIB1的细胞质免疫染色均为阴性。由于这种不寻常的染色模式，我们对所有病例重复进行了染色，发现结果具有可重复性。因此，我们认为MIB1的细胞质免疫染色阳性是透明变梁状腺瘤的一个特征性表现，有助于将其与其他甲状腺肿瘤区分开来。
