McCluggage W G, Maxwell P, McBride H A, Hamilton P W, Bharucha H
Department of Histopathology, Royal Group of Hospitals, Belfast, Northern Ireland.
Int J Gynecol Pathol. 1995 Jul;14(3):209-16. doi: 10.1097/00004347-199507000-00003.
This study was undertaken to determine whether immunostaining with the monoclonal antibodies Ki-67 and MIB1 is of use in the distinction of tuboendometrial metaplasia from endocervical adenocarcinoma and adenocarcinoma in situ (AIS) on formalin-fixed, paraffin-embedded tissue. Tissue sections from 100 cases (52 normal endocervix, 27 endocervical tuboendometrial metaplasia, eight endocervical AIS, 13 endocervical adenocarcinoma) were stained with Ki-67 and MIB1 after microwave pretreatment in citrate buffer. Ki-67 and MIB1 labelling indices (LIs) were calculated in each case by determining the percentage of positive-staining nuclei. Five hundred nuclei were counted in each case. Significant differences in Ki-67 and MIB1 LIs existed between the adenocarcinoma group and the tuboendometrial metaplasia group and between the AIS group and the tuboendometrial metaplasia group. No significant differences existed between the adenocarcinoma and AIS groups. Over all groups, the Ki-67 LI was consistently lower than the MIB1 LI. The results indicate that immunohistochemical staining with Ki-67 and MIB1 may be of use in the often difficult histological distinction of tuboendometrial metaplasia from malignant endocervical glandular lesions.
本研究旨在确定在福尔马林固定、石蜡包埋的组织上，使用单克隆抗体Ki-67和MIB1进行免疫染色是否有助于区分输卵管内膜化生与宫颈管腺癌及原位腺癌（AIS）。对100例病例（52例正常宫颈管、27例宫颈管输卵管内膜化生、8例宫颈管AIS、13例宫颈管腺癌）的组织切片在柠檬酸盐缓冲液中进行微波预处理后，用Ki-67和MIB1进行染色。通过确定阳性染色细胞核的百分比，计算每例病例的Ki-67和MIB1标记指数（LIs）。每例病例计数500个细胞核。腺癌组与输卵管内膜化生组之间以及AIS组与输卵管内膜化生组之间的Ki-67和MIB1 LIs存在显著差异。腺癌组与AIS组之间无显著差异。在所有组中，Ki-67 LI始终低于MIB1 LI。结果表明，Ki-67和MIB1免疫组化染色可能有助于在组织学上区分常常难以鉴别的输卵管内膜化生与宫颈管恶性腺性病变。
