引起牛乳腺炎的金黄色葡萄球菌分离株中凝固酶基因多态性的患病率。
Prevalence of coagulase gene polymorphism in Staphylococcus aureus isolates causing bovine mastitis.
作者信息
Aarestrup F M, Dangler C A, Sordillo L M
机构信息
National Veterinary Laboratory, Department of Microbiology, Copenhagen, Denmark.
出版信息
Can J Vet Res. 1995 Apr;59(2):124-8.
Abstract
This study was conducted to investigate polymorphism of the coagulase gene of Staphylococcus aureus causing bovine mastitis. One hundred eighty-seven strains of S. aureus were isolated from bovine mastitic milk samples obtained from 187 different Danish dairy farms. The isolates were characterised for restriction fragment length polymorphism (RFLP) of the coagulase gene. A variable region of the coagulase gene was amplified using the polymerase chain reaction (PCR) followed by AluI restriction enzyme digestion. A total of 15 different RFLP patterns were observed. The predominant pattern was found in 35% of the isolates. The ease of analysing coagulase gene polymorphisms among a large number of strains, and the multiple distinct polymorphic patterns generated, supports the use of this technique in epidemiological investigations of bovine mastitis. The predominating variants may have predelection for causing intramammary infections.
摘要
本研究旨在调查引起奶牛乳房炎的金黄色葡萄球菌凝固酶基因的多态性。从丹麦187个不同奶牛场采集的奶牛乳房炎乳样中分离出187株金黄色葡萄球菌。对分离株进行凝固酶基因的限制性片段长度多态性（RFLP）分析。利用聚合酶链反应（PCR）扩增凝固酶基因的可变区，随后用AluI限制性内切酶消化。共观察到15种不同的RFLP模式。35%的分离株中发现了主要模式。在大量菌株中分析凝固酶基因多态性的简便性以及产生的多种不同多态性模式，支持将该技术用于奶牛乳房炎的流行病学调查。占主导地位的变体可能更容易引起乳腺内感染。
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