Rodrigues da Silva Elizabete, da Silva Nivaldo
Laboratory of Mastitis Control, Veterinary School - Federal University of Minas Gerais/UFMG, Belo Horizonte, Minas Gerais, Brazil.
Can J Vet Res. 2005 Oct;69(4):260-4.
A typing procedure based on polymorphism of the coagulase gene (coa) was used to discriminate Staphylococcus aureus isolated from Minas Gerais dairy cows with mastitis. Amplification of the gene from the 64 S. aureus isolates produced 27 different polymerase chain reaction (PCR) products; 60 isolates showed only 1 amplicon, and 4 showed 2 amplicons. The isolates were grouped into 49 types by analyzing the restriction fragment length polymorphism (RFLP) of the coa gene; the 10 most common types accounted for 39% of the isolates. The results demonstrate that many variants of the coa gene are present in the studied region, although only a few predominate.
一种基于凝固酶基因（coa）多态性的分型方法被用于鉴别从米纳斯吉拉斯州患乳腺炎的奶牛中分离出的金黄色葡萄球菌。从64株金黄色葡萄球菌分离株中扩增该基因产生了27种不同的聚合酶链反应（PCR）产物；60株分离株仅显示1个扩增子，4株显示2个扩增子。通过分析coa基因的限制性片段长度多态性（RFLP），将分离株分为49种类型；10种最常见的类型占分离株的39%。结果表明，尽管只有少数类型占主导，但在所研究区域存在许多coa基因变体。
