Takikawa H, Nishikawa K, Sano N, Yamanaka M, Horie T
Department of Medicine, Teikyo University School of Medicine, Tokyo, Japan.
Dig Dis Sci. 1995 Aug;40(8):1792-7. doi: 10.1007/BF02212704.
Biliary excretion of lithocholate-3-sulfate is markedly impaired in EHBR. To examine the mechanism of biliary lithocholate-3-sulfate excretion in EHBR, the effects of colchicine treatment, a vesicular transport inhibitor, and infusion of taurocholate and organic anions were studied in EHBR and Sprague-Dawley rats. Colchicine treatment and taurocholate infusion had no effect of biliary lithocholate-3-sulfate excretion in EHBR, suggesting that biliary lithocholate-3-sulfate excretion is not mediated by the vesicular transport or by the bile acid excretory pathway. In control Sprague-Dawley rats, both sulfobromophthalein and dibromosulfophthalein infusion inhibited biliary lithocholate-3-sulfate excretion. In contrast, in EHBR dibromosulfophthalein infusion inhibited biliary lithocholate-3-sulfate excretion but BSP infusion did not. Indocyanine green and pravastatin infusion did not affect biliary lithocholate-3-sulfate excretion but pravastatin infusion had no effect in EHBR. These findings indicate that, whether physiologically important or not, two of more excretory pathways for organic anions exist at the canalicular membrane other than the ATP-dependent one.
在EHBR中，石胆酸-3-硫酸盐的胆汁排泄明显受损。为了研究EHBR中胆汁石胆酸-3-硫酸盐排泄的机制，在EHBR和Sprague-Dawley大鼠中研究了秋水仙碱治疗（一种囊泡转运抑制剂）、牛磺胆酸盐输注和有机阴离子输注的影响。秋水仙碱治疗和牛磺胆酸盐输注对EHBR中胆汁石胆酸-3-硫酸盐的排泄没有影响，这表明胆汁石胆酸-3-硫酸盐的排泄不是由囊泡转运或胆汁酸排泄途径介导的。在对照Sprague-Dawley大鼠中，磺溴酞钠和二溴磺酞钠输注均抑制胆汁石胆酸-3-硫酸盐的排泄。相比之下，在EHBR中，二溴磺酞钠输注抑制胆汁石胆酸-3-硫酸盐的排泄，但磺溴酞钠输注则无此作用。吲哚菁绿和普伐他汀输注不影响胆汁石胆酸-3-硫酸盐的排泄，但普伐他汀输注对EHBR无影响。这些发现表明，无论生理上是否重要，除了ATP依赖性途径外，在胆小管膜上还存在两种或更多种有机阴离子排泄途径。
