Tikkanen H O, Näveri H K, Härkönen M H
Department of Clinical Chemistry, University of Helsinki, Finland.
Eur J Appl Physiol Occup Physiol. 1995;70(4):281-7. doi: 10.1007/BF00865023.
The effect of progressive, low-intensity endurance training on regulatory enzyme activities in slow-twitch (ST) and fast-twitch (FT) muscle fibres was studied in 32 rats. Of those rats 16 were trained on a treadmill at a running speed of 10 m.min-1 5 days a week over an 8-week period. Running time was progressively increased from 15 min to 2 h.day-1. Of the rats 4 trained and 4 sedentary rats were also subjected to acute exhausting exercise. Enzyme activities of phosphofructokinase 1 (PFK1) from glycolysis, alpha-ketoglutarate dehydrogenase (alpha-KGDH) from the Krebs cycle and carnitine palmitoyltransferase (CPT I and II) from fatty acid metabolism in soleus, tibialis anterior and gastrocnemius muscles were measured in trained and sedentary rats. Enzyme activities of individual ST and FT fibres were measured from the freeze-dried gastrocnemius muscle of 8 trained and 8 sedentary rats. In the sedentary rats the activity of PFK1 in tibialis anterior and soleus muscles was 141% and 41% of the activity in gastrocnemius muscle, respectively. The activity of alpha-KGDH in tibialis anterior and soleus muscles was 164% and 278% of the activity in gastrocnemius muscle, respectively. The activity of CPT I in tibialis anterior and gastrocnemius muscles were at the same level, but in soleus muscle the activity was 127% of that in mixed muscle. Endurance training increased enzyme activities of alpha-KGDH and CPT I significantly (P < 0.05) in gastrocnemius muscle but not in soleus or tibialis anterior muscle.(ABSTRACT TRUNCATED AT 250 WORDS)
在32只大鼠中研究了渐进性低强度耐力训练对慢肌纤维（ST）和快肌纤维（FT）中调节酶活性的影响。其中16只大鼠在跑步机上以10米/分钟的速度每周训练5天，持续8周。跑步时间从每天15分钟逐渐增加到2小时。4只训练大鼠和4只 sedentary大鼠也进行了急性力竭运动。在训练大鼠和 sedentary大鼠中，测量了比目鱼肌、胫骨前肌和腓肠肌中糖酵解的磷酸果糖激酶1（PFK1）、三羧酸循环的α-酮戊二酸脱氢酶（α-KGDH）以及脂肪酸代谢的肉碱棕榈酰转移酶（CPT I和II）的酶活性。从8只训练大鼠和8只 sedentary大鼠的冻干腓肠肌中测量了单个ST和FT纤维的酶活性。在 sedentary大鼠中，胫骨前肌和比目鱼肌中PFK1的活性分别是腓肠肌中活性的141%和41%。胫骨前肌和比目鱼肌中α-KGDH的活性分别是腓肠肌中活性的164%和278%。胫骨前肌和腓肠肌中CPT I的活性处于同一水平，但比目鱼肌中的活性是混合肌中的127%。耐力训练使腓肠肌中α-KGDH和CPT I的酶活性显著增加（P<0.05），但比目鱼肌或胫骨前肌中没有增加。（摘要截断于250字）
