Jones H C, Harris N G, Briggs R W, Williams S C
Department of Pharmacology and Therapeutics, University of Florida, Gainesville, USA.
Exp Neurol. 1995 Jun;133(2):144-52. doi: 10.1006/exnr.1995.1017.
The H-Tx rat has inherited hydrocephalus with an onset in late gestation. Ventriculosubcutaneous shunts were placed in a group of hydrocephalic pups at 3-6 days after birth and in another group at 8-12 days after birth. Multislice proton magnetic resonance (MR) images were taken of shunt-treated pups at 7, 14, or 21 days and of age-matched control and untreated hydrocephalic rats and were subjected to quantitative analysis. Some rats were also imaged before surgery. The volume of the ventricles of untreated hydrocephalic pups increased linearly with age at a rate of 52 microliters/day. The ventricles of shunted pups were reduced from the preshunt condition and were around 20% of age-matched hydrocephalic rats. There was no significant difference in the post-shunt volume between early and late shunt groups. The cortical mantle thickness in both groups of shunt-treated rats was significantly thicker than in untreated pups and close to that of control rats. It is concluded that shunt treatment at both ages reversed the hydrocephalus as measured from MR images, although other evidence from fixed brains suggests that normal morphology may not be achieved at the cellular level even for early shunts.
H-Tx大鼠遗传性脑积水于妊娠晚期发病。在出生后3 - 6天给一组脑积水幼崽植入脑室-皮下分流管，另一组在出生后8 - 12天植入。在7、14或21天时对接受分流治疗的幼崽以及年龄匹配的对照和未治疗的脑积水大鼠进行多层质子磁共振（MR）成像，并进行定量分析。一些大鼠在手术前也进行了成像。未治疗的脑积水幼崽脑室体积随年龄呈线性增加，速率为52微升/天。接受分流的幼崽脑室体积较分流前减小，约为年龄匹配的脑积水大鼠的20%。早期和晚期分流组分流后的体积无显著差异。两组接受分流治疗的大鼠的皮质被膜厚度均显著厚于未治疗的幼崽，且接近对照大鼠。结论是，从MR图像测量来看，两个年龄段的分流治疗均能逆转脑积水，尽管固定脑的其他证据表明，即使是早期分流，在细胞水平也可能无法实现正常形态。
