H-Tx大鼠脑积水模型中淋巴系统对脑脊液吸收减少的证据。
Evidence for reduced lymphatic CSF absorption in the H-Tx rat hydrocephalus model.
作者信息
Rammling Matthias, Madan Meenu, Paul Leena, Behnam Babak, Pattisapu Jogi V
机构信息
Hydrocephalus Research Laboratory, Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, FL 32816, USA.
出版信息
Cerebrospinal Fluid Res. 2008 Oct 16;5:15. doi: 10.1186/1743-8454-5-15.
BACKGROUND
There is mounting evidence that spinal fluid absorption takes place not only at the arachnoid villi, but also at several extracranial sites, which might serve as a reserve mechanism for, or be primarily involved in the absorption of CSF in hydrocephalus.
METHODS
We compared the nasal lymphatic pathway in congenital Hydrocephalus-Texas (H-Tx) rats in unaffected and affected hydrocephalic (HC) siblings with that of control Sprague Dawley (SD) rat pups. The animals were examined after immediate post mortem injection of Evan's blue dye into the cisterna magna at 6 and 10 days of age. The specimens were evaluated for amount of dye penetration into the nasal passages.
RESULTS
We found more dye visualization in the olfactory regions of control SD (14/16 at P6, 14/16 at P10) and unaffected H-Tx (13/17 at P6, 13/16 at P10) compared with HC animals (0/14 at P6, 3/15 at P10). This difference was more pronounced at 10 days of age. The dye was not visualized in the cervical lymph nodes or venous channels in these acute experiments.
CONCLUSION
The results of this study suggest that nasal lymphatic cerebrospinal fluid absorption is reduced in the H-Tx rat hydrocephalus model.
背景
越来越多的证据表明，脑脊液吸收不仅发生在蛛网膜绒毛，还发生在几个颅外部位，这些部位可能作为一种储备机制，或者在脑积水时主要参与脑脊液的吸收。
方法
我们比较了先天性脑积水-德州（H-Tx）大鼠未受影响和受影响的脑积水（HC）同窝幼崽与对照斯普拉格-道利（SD）大鼠幼崽的鼻淋巴途径。在6日龄和10日龄时，在动物死后立即将伊文思蓝染料注入枕大池后进行检查。评估标本中染料渗透到鼻腔的量。
结果
我们发现，与HC动物（6日龄时0/14，10日龄时3/15）相比，对照SD大鼠（6日龄时14/16，10日龄时14/16）和未受影响的H-Tx大鼠（6日龄时13/17，10日龄时13/16）的嗅觉区域有更多的染料可见。这种差异在10日龄时更为明显。在这些急性实验中，染料在颈淋巴结或静脉通道中不可见。
结论
本研究结果表明，H-Tx大鼠脑积水模型中鼻淋巴脑脊液吸收减少。
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