Pinaev G, Schutt C E, Lindberg U
Department of Cell Culture, Institute of Cytology Russian Academy of Sciences, St. Petersburg.
FEBS Lett. 1995 Aug 7;369(2-3):144-8. doi: 10.1016/0014-5793(95)00724-n.
Actin polymerization has been studied in the absence of excess nucleotide. Using G-actin ATP monomers, it was shown that mechanical shearing stimulates ATP hydrolysis. The procedures used enabled the detection of differential effects of phalloidin and tetramethylrhodamine-phalloidin, on the P(i)-release step of the actin ATPase. It is concluded that tetramethylrhodamine, in contrast to phalloidin, accelerates P(i)-release from actin filaments.
在没有过量核苷酸的情况下对肌动蛋白聚合进行了研究。使用G-肌动蛋白ATP单体，研究表明机械剪切会刺激ATP水解。所采用的实验步骤能够检测鬼笔环肽和四甲基罗丹明-鬼笔环肽对肌动蛋白ATP酶的无机磷酸释放步骤的不同影响。得出的结论是，与鬼笔环肽相反，四甲基罗丹明会加速肌动蛋白丝中无机磷酸的释放。
