Vissing H, Meyer W K, Aagaard L, Tommerup N, Thiesen H J
Basel Institute for Immunology, Switzerland.
FEBS Lett. 1995 Aug 7;369(2-3):153-7. doi: 10.1016/0014-5793(95)00728-r.
We report the characterization of three novel members of the KRAB-domain containing C2-H2 zinc finger family (ZNF133, 136 and 140). KRAB (Krüppel-associated box) is an evolutionarily conserved protein domain found N-terminally with respect to the zinc finger repeats that encodes the DNA binding domain. ZNF133 and ZNF140 have both the KRAB A- and KRAB B-boxes present at their N-terminus, whereas ZNF136 contains only the KRAB A-box. We have previously demonstrated that the KRAB domains derived from ZNF133 and ZNF140 are potent transcriptional repression domains [Margolin et al. (1994) Proc. Natl. Acad. Sci. USA 91, 4509-4513]. The KRAB domain from ZNF136, containing only subdomain A, is a considerable weaker suppression domain; however, when fused to the heterologous KRAB B subdomain of ZNF10 (KOX1) the two subdomains from a KRAB domain which induces repression as potently as previously reported KRAB domains.
我们报告了含KRAB结构域的C2-H2锌指家族（ZNF133、136和140）三个新成员的特征。KRAB（Krüppel相关盒）是一个在进化上保守的蛋白质结构域，位于编码DNA结合结构域的锌指重复序列的N端。ZNF133和ZNF140在其N端均存在KRAB A盒和KRAB B盒，而ZNF136仅包含KRAB A盒。我们之前已经证明，源自ZNF133和ZNF140的KRAB结构域是有效的转录抑制结构域[Margolin等人（1994年）《美国国家科学院院刊》91，4509 - 4513]。来自ZNF136的KRAB结构域仅包含A亚结构域，是一个相当弱得多的抑制结构域；然而，如果与ZNF10（KOX1）的异源KRAB B亚结构域融合，这两个亚结构域就形成一个与之前报道的KRAB结构域一样能有效诱导抑制作用的KRAB结构域。
