Sotiropoulos A, Moutoussamy S, Binart N, Kelly P A, Finidori J
INSERM U344, Faculté de Médecine Necker, Paris, France.
FEBS Lett. 1995 Aug 7;369(2-3):169-72. doi: 10.1016/0014-5793(95)00734-q.
Growth hormone receptor (GHR) signaling involves activation of the Janus Kinases (Jak) and of Stat proteins (signal transducers and activators of transcription). Growth hormone (GH) induces transcriptional activation of c-fos gene and the c-sis inducible element (SIE) of its promoter was shown to bind the Stat proteins. Using cells co-transfected with GHR and Stat 3 expression vectors, we directly demonstrate that GH induces tyrosine phosphorylation of Stat 3 and its binding to the SIE probe. We showed, using mutant forms of GHR, that only the cytoplasmic membrane proximal domain of the receptor, including a conserved proline rich region (box 1), is required for this effect.
生长激素受体（GHR）信号传导涉及Janus激酶（Jak）和Stat蛋白（信号转导子和转录激活子）的激活。生长激素（GH）诱导c-fos基因的转录激活，并且其启动子的c-sis诱导元件（SIE）已被证明可结合Stat蛋白。利用共转染了GHR和Stat 3表达载体的细胞，我们直接证明了GH诱导Stat 3的酪氨酸磷酸化及其与SIE探针的结合。我们使用GHR的突变形式表明，仅受体的细胞质膜近端结构域，包括一个保守的富含脯氨酸区域（框1），对此效应是必需的。
