Alfandari D, Whittaker C A, DeSimone D W, Darribère T
U.R.A. CNRS 1135, Laboratoire de Biologie Moléculaire et Cellulaire du Développement, Université P. et M. Curie, Paris, France.
Dev Biol. 1995 Aug;170(2):249-61. doi: 10.1006/dbio.1995.1212.
Mesodermal cell migration during amphibian gastrulation is dependent on cellular interactions with fibronectin. One mechanism whereby cells bind fibronectin is through alpha v-containing integrin heterodimers. In order to investigate the role of alpha v in amphibian gastrulation, we have cloned the Pleurodeles homologue of the integrin alpha v subunit using homology PCR. The deduced amino acid sequence is 73 and 74% identical with the human and chick homologues, respectively. The 4.8-kb mRNA is expressed during oogenesis and persists throughout development. Messenger RNA and protein are widely expressed in oocytes and embryos while cell surface expression is spatially regulated. The protein first appears on the plasma membrane of fully grown oocytes. Fertilization results in the progressive loss of alpha v membrane localization. Before and during gastrulation, the integrin alpha v subunit is expressed on the surface of mesodermal cells. These data show that alpha v expression is developmentally regulated by a post-translational mechanism which correlates with the onset of mesodermal cell migration at gastrulation.
两栖类原肠胚形成过程中中胚层细胞的迁移依赖于细胞与纤连蛋白的相互作用。细胞结合纤连蛋白的一种机制是通过含αv的整合素异二聚体。为了研究αv在两栖类原肠胚形成中的作用，我们利用同源PCR克隆了整合素αv亚基的非洲爪蟾同源物。推导的氨基酸序列与人类和鸡的同源物分别有73%和74%的同一性。4.8kb的mRNA在卵子发生过程中表达，并在整个发育过程中持续存在。信使RNA和蛋白质在卵母细胞和胚胎中广泛表达，而细胞表面表达则受到空间调控。该蛋白质首先出现在完全成熟卵母细胞的质膜上。受精导致αv膜定位的逐渐丧失。在原肠胚形成之前和期间，整合素αv亚基在中胚层细胞表面表达。这些数据表明，αv的表达受翻译后机制的发育调控，该机制与原肠胚形成时中胚层细胞迁移的开始相关。
