Moreau N, Aumais J P, Prudhomme C, Morris S M, Yu-Lee L Y
Groupe Développement des Vertébrés, UMR 7622 CNRS-Université Pierre et Marie Curie, Paris, France.
Int J Dev Biol. 2001 Oct;45(7):839-43.
To identify gene products important for gastrulation in the amphibian Pleurodeles waltl, a screen for regional differences in new protein expression at the early gastrula stage was performed. A 45 kDa protein whose synthesis was specific for progenitor endodermal cells was identified. Microsequencing and cDNA cloning showed that P45 is highly homologous to rat NUDC, a protein suggested to play a role in nuclear migration. Although PNUDC can be detected in all regions of the embryo, its de novo synthesis is tightly regulated spatially and temporally throughout oogenesis and embryonic development. New PNUDC synthesis in the progenitor endodermal cells depends on induction by the mesodermal cells in the gastrula. During development, PNUDC is localized in the egg cortical cytoplasm, at the cleavage furrow during the first embryonic division, around the nuclei and cortical regions of bottle cells in the gastrula, and at the basal region of polarized tissues in the developing embryo. These results show for the first time the expression and compartmentalization of PNUDC at distinct stages during amphibian development.
为了鉴定对有尾两栖动物肋突螈原肠胚形成重要的基因产物，我们对原肠胚早期新蛋白质表达的区域差异进行了筛选。鉴定出一种45 kDa的蛋白质，其合成对祖内胚层细胞具有特异性。微量测序和cDNA克隆表明，P45与大鼠NUDC高度同源，后者是一种被认为在核迁移中起作用的蛋白质。虽然在胚胎的所有区域都能检测到PNUDC，但其从头合成在整个卵子发生和胚胎发育过程中受到严格的时空调控。祖内胚层细胞中PNUDC的新合成依赖于原肠胚中胚层细胞的诱导。在发育过程中，PNUDC定位于卵皮质细胞质、第一次胚胎分裂时的分裂沟、原肠胚中瓶状细胞的细胞核和皮质区域周围，以及发育中胚胎极化组织的基部区域。这些结果首次展示了PNUDC在两栖动物发育不同阶段的表达和分隔。
