Arpaia G, Carattoli A, Macino G
Dipartimento di Biopatologia Umana, Policlinico Umberto I, Università di Roma La Sapienza, Italy.
Dev Biol. 1995 Aug;170(2):626-35. doi: 10.1006/dbio.1995.1242.
The albino-3 gene of Neurospora crassa codes for geranylgeranyl pyrophosphate synthase, an enzyme involved in the biosynthesis of carotenoids. The albino-3 locus encodes two overlapping transcription units that give rise to two mRNAs of 2.2 kb (al-3(c)) and 1>6 kb (al-3(m)), with the promoter of the latter residing in the transcribed region of the former. The 1.6-kb transcript was transiently expressed in the mycelium after light induction, while the 2.2-kb mRNA appeared in conidiating cultures only, independently of light. Al-3(c) mRNA was enhanced in the conidiophores and was impaired in mutants blocked at the early stages of conidiation; al-3(c) therefore represents a conidiation-specific transcript. The al-3(c) mRNA level increased upon illumination with blue light and fluctuated according to the circadian cycle.
粗糙脉孢菌的白化基因-3编码香叶基香叶基焦磷酸合酶，这是一种参与类胡萝卜素生物合成的酶。白化基因-3位点编码两个重叠的转录单元，产生2.2 kb（al-3(c)）和1.6 kb（al-3(m)）的两种mRNA，后者的启动子位于前者的转录区域。1.6 kb的转录本在光诱导后在菌丝体中短暂表达，而2.2 kb的mRNA仅出现在产孢培养物中，与光无关。Al-3(c) mRNA在分生孢子梗中增强，在分生孢子形成早期受阻的突变体中受损；因此，al-3(c)代表一种分生孢子形成特异性转录本。al-3(c) mRNA水平在蓝光照射下增加，并根据昼夜节律周期波动。
