Cimprich P, Slavík J, Kotyk A
Department of Membrane Transport, CzAcadSci, Prague, Czech Republic.
FEMS Microbiol Lett. 1995 Aug 1;130(2-3):245-51. doi: 10.1111/j.1574-6968.1995.tb07727.x.
Fluorescence ratio imaging microscopy using pH-sensitive fluorescent dyes makes it possible to evaluate statistical distribution of intracellular pH in a population of the yeast S. cerevisiae examined in a thin layer of suspension in a Petri dish. The distribution appears to fit a Gaussian curve with a half-width around the 0.4 pH unit. The curve became slightly narrower after resuspension in a strong buffer; the mean values shifted with the pH of the buffer. The shape of the distribution curves of both resting and growing cells in various phases of growth does not change significantly. Likewise, addition of 1% of glucose, 50 microM suloctidil or 100 microM diethylstilbestrol brings about no alteration. The only value which clearly changes is the average cytoplasmic pH.
使用对pH敏感的荧光染料进行荧光比率成像显微镜检查，可以评估在培养皿中薄层悬浮液中检测的酿酒酵母群体中细胞内pH的统计分布。该分布似乎符合高斯曲线，半峰宽约为0.4个pH单位。在强缓冲液中重悬后，曲线略微变窄；平均值随缓冲液的pH值而变化。在不同生长阶段，静止细胞和生长细胞的分布曲线形状没有明显变化。同样，添加1%的葡萄糖、50微摩尔舒洛地尔或100微摩尔己烯雌酚也不会引起改变。唯一明显变化的值是平均细胞质pH值。
