Nowak A, Kur J
Department of Microbiology, Technical University of Gdańsk, Poland.
FEMS Microbiol Lett. 1995 Aug 1;130(2-3):327-32. doi: 10.1111/j.1574-6968.1995.tb07739.x.
The recA gene has been used as a target in screening for the presence of acinetobacters on the genospecies level and differentiation of relevant acinetobacter species from one another by PCR. Primers deduced from known recA gene sequences of Acinetobacter calcoaceticus and Neisseria gonorrhoeae allowed the amplification of DNAs from all Acinetobacter genospecies. The size of the amplified DNA fragment from all genospecies tested was approximately 435-500 bp relative to DNA size markers. The amplified products were examined further by restriction fragment length polymorphism (RFLP) analysis. Restriction analysis with only two enzymes, MboI and HinfI, enabled us to identify all known genospecies. Since this method uses conserved recA gene sequences for primers, it is expected to be applicable for the identification of most bacterial species.
recA基因已被用作在基因种水平上筛选不动杆菌的存在以及通过PCR区分相关不动杆菌物种的靶标。从醋酸钙不动杆菌和淋病奈瑟菌已知的recA基因序列推导的引物能够扩增所有不动杆菌基因种的DNA。相对于DNA大小标记物，所有测试基因种的扩增DNA片段大小约为435 - 500 bp。通过限制性片段长度多态性（RFLP）分析对扩增产物进行进一步检测。仅用两种酶MboI和HinfI进行限制性分析就能使我们鉴定所有已知基因种。由于该方法使用保守的recA基因序列作为引物，预计它可用于鉴定大多数细菌物种。
