Jawad A, Snelling A M, Heritage J, Hawkey P M
Department of Microbiology, University of Leeds, UK.
FEMS Microbiol Lett. 1998 Aug 15;165(2):357-62. doi: 10.1111/j.1574-6968.1998.tb13170.x.
The genus Acinetobacter is subdivided into genospecies on the basis of DNA relatedness of strains. Phenotypic tests are insufficient to identify the genospecies to which an isolate belongs. The effectiveness of two previously described PCR-based methods for genospeciating Acinetobacter spp. was compared using a group of 32 well-characterised strains representing six genospecies. Amplified ribosomal DNA restriction analysis (ARDRA) correctly identified all 32 strains. Using restriction fragment length polymorphism (RFLP) of recA PCR amplimers, only six of the 32 strains were correctly identified. Heterogeneity in the recA gene sequence was demonstrated within five of the genospecies. ARDRA proved to be a reliable method whereas analysis of recA RFLP profiles did not enable the genospecies of most of the isolates of Acinetobacter spp. to be determined.
不动杆菌属根据菌株的DNA相关性被细分为基因种。表型试验不足以鉴定分离株所属的基因种。使用一组代表六个基因种的32株特征明确的菌株，比较了两种先前描述的基于PCR的不动杆菌属基因分型方法的有效性。扩增核糖体DNA限制性分析（ARDRA）正确鉴定了所有32株菌株。使用recA PCR扩增子的限制性片段长度多态性（RFLP），32株菌株中只有6株被正确鉴定。在五个基因种中证明了recA基因序列的异质性。ARDRA被证明是一种可靠的方法，而recA RFLP图谱分析不能确定大多数不动杆菌属分离株的基因种。
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