Panasenko O M, Evgina S A, Driomina E S, Sharov V S, Sergienko V I, Vladimirov Y A
Research Institute of Physico-Chemical Medicine, Moscow, Russia.
Free Radic Biol Med. 1995 Aug;19(2):133-40. doi: 10.1016/0891-5849(94)00211-2.
The accumulation of lipid peroxidation products reacting with 2-thiobarbituric acid (TBARS) has been observed both in very low density blood lipoprotein (VLDL) and suspensions of liposomes prepared from VLDL phospholipids incubated with hypochlorite. Butylated hydroxytoluene (BHT) completely inhibited TBARS formation at a concentration of 100 microM, at which it decreased the concentration of hypochlorite in the absence of liposomes only by 7%. The formation of lipid peroxidation products in course of the incubation of egg yolk phospholipid liposomes with hypochlorite has been revealed using three methods: (1) measurement of TBARS, (2) measurement of additional amounts of TBARS resulting from the introduction of excess Fe2+ to peroxidized liposomes (delta TBARS), and (3) measurement of the chemiluminescence flash amplitude appeared upon the addition of Fe2+ to the suspension. The results obtained by all these methods were similar: Lipid peroxidation products were accumulated during the first 2 to 3 h of liposome incubation with 100 microM hypochlorite, and the amount of lipid peroxidation products accumulated after incubation was directly proportional to the initial hypochlorite concentration. These data suggest that hypochlorite can initiate lipid peroxidation both in lipoproteins and phospholipid liposomes.
在极低密度血液脂蛋白（VLDL）以及由VLDL磷脂制备的脂质体悬浮液（与次氯酸盐一起孵育）中，均观察到了与2-硫代巴比妥酸（TBARS）发生反应的脂质过氧化产物的积累。丁基羟基甲苯（BHT）在浓度为100微摩尔时完全抑制了TBARS的形成，在此浓度下，它仅在不存在脂质体时使次氯酸盐浓度降低了7%。利用三种方法揭示了蛋黄磷脂脂质体与次氯酸盐孵育过程中脂质过氧化产物的形成：（1）TBARS的测量；（2）向过氧化脂质体中引入过量Fe2+后产生的额外TBARS量（δTBARS）的测量；（3）向悬浮液中添加Fe2+时出现的化学发光闪光幅度的测量。通过所有这些方法获得的结果相似：脂质体与100微摩尔次氯酸盐孵育的前2至3小时内积累了脂质过氧化产物，孵育后积累的脂质过氧化产物量与初始次氯酸盐浓度成正比。这些数据表明，次氯酸盐可在脂蛋白和磷脂脂质体中引发脂质过氧化。
