Kotwani A, Mehta V L, Iyengar B
Department of Pharmacology, Maulana Azad Medical College, New Delhi.
Indian J Physiol Pharmacol. 1995 Apr;39(2):131-4.
Aspirin (acetylsalicyclic acid) was dissolved either in normal saline or in phosphate buffer and was used in two doses to find out whether teratogenic potential of aspirin in chick blastoderm model is due to its acidic property or due to drug action. Drug was injected sub-blastodermally by window technique in fresh embryonated eggs after 17 hours of incubation at 39 degrees C. Eggs were re-incubated and harvested at 40 hours. Normal development of embryos was seen with normal saline and percentage of normal embryos with 30 micrograms (pH-3.19) and 120 micrograms (pH-2.64) aspirin was 31.7 and 4.9 respectively. Buffer produced 80.8% normal embryos and buffered 30 micrograms (pH-6.87) and 120 micrograms (pH-6.69) aspirin produced 67.7% and 30.8% normal embryos respectively. Changing the pH of aspirin to near neutral decreased the defect induced by aspirin but a significant effect of aspirin was observed at higher dose which could be independent of pH action.
阿司匹林（乙酰水杨酸）分别溶解于生理盐水或磷酸盐缓冲液中，并以两种剂量使用，以确定阿司匹林在鸡胚盘模型中的致畸潜力是由于其酸性性质还是药物作用。在39摄氏度孵育17小时后，通过开窗技术将药物皮下注射到新鲜的受精鸡蛋胚盘下。鸡蛋重新孵育，并在40小时时收获。用生理盐水观察到胚胎正常发育，30微克（pH值为3.19）和120微克（pH值为2.64）阿司匹林处理后的正常胚胎百分比分别为31.7%和4.9%。缓冲液产生80.8%的正常胚胎，缓冲后的30微克（pH值为6.87）和120微克（pH值为6.69）阿司匹林分别产生67.7%和30.8%的正常胚胎。将阿司匹林的pH值调至接近中性可减少阿司匹林诱导的缺陷，但在较高剂量下观察到阿司匹林有显著作用，这可能与pH值作用无关。
