Götz W, Osmers R, Herken R
Zentrum Anatomie , Abteilung Histologie, Göttingen, Germany.
J Anat. 1995 Feb;186 ( Pt 1)(Pt 1):111-21.
The unsegmented axial mesenchyme is the first anlage of the embryonic vertebral column in amniotes. Its development and differentiation are influenced by the notochord. The appearance of extracellular matrix (ECM) components which may regulate the developmental processes in the human embryonic axial mesenchyme and the notochord was studied in 5 human embryos between Carnegie stages 12 and 14 (4th and early 5th gestational wk) by using Alcian blue histochemistry and immunohistochemistry with antibodies against types I and II collagens, the proteoglycans aggrecan, biglycan and decorin, the glycoproteins fibronectin, laminin and tenascin and the growth factor TGF-beta. The results demonstrate that the notochordal sheath contains high amounts of sulphated glycosaminoglycans, hyaluronic acid and the glycoproteins fibronectin, laminin and tenascin during all developmental stages examined. Additionally type II collagen and the small proteoglycan decorin were found. Type I collagen and the small proteoglycan biglycan could be detected in notochordal cells. The number of TGF-beta-immunoreactive notochordal cells increased between stages 12 and 14. The ECM of the axial mesenchyme is rich in sulphated glycosaminoglycans, hyaluronic acid, fibronectin and tenascin. Strong immunoreactions for collagen type I were found only during the early stage 12. The absence of aggrecan and keratan sulphate indicates that large aggregating proteoglycan complexes are missing. Epithelial-mesenchymal interactions which may be influenced by TGF-beta and the ECM components demonstrated are discussed.
在羊膜动物中，未分段的轴旁间充质是胚胎脊柱的首个原基。其发育和分化受脊索影响。运用阿尔新蓝组织化学和针对I型和II型胶原蛋白、蛋白聚糖聚集蛋白聚糖、双糖链蛋白聚糖和核心蛋白聚糖、糖蛋白纤连蛋白、层粘连蛋白和腱生蛋白以及生长因子转化生长因子-β（TGF-β）的抗体进行免疫组织化学，研究了卡内基分期12至14期（妊娠第4周和第5周早期）的5个人类胚胎中可能调节人类胚胎轴旁间充质和脊索发育过程的细胞外基质（ECM）成分的出现情况。结果表明，在所检查的所有发育阶段，脊索鞘都含有大量硫酸化糖胺聚糖、透明质酸以及糖蛋白纤连蛋白、层粘连蛋白和腱生蛋白。此外，还发现了II型胶原蛋白和小蛋白聚糖核心蛋白聚糖。在脊索细胞中可检测到I型胶原蛋白和小蛋白聚糖双糖链蛋白聚糖。在分期12至14期之间，TGF-β免疫反应性脊索细胞的数量增加。轴旁间充质的ECM富含硫酸化糖胺聚糖、透明质酸、纤连蛋白和腱生蛋白。仅在12期早期发现了I型胶原蛋白的强免疫反应。聚集蛋白聚糖和硫酸角质素的缺失表明大型聚集蛋白聚糖复合物缺失。讨论了可能受TGF-β和所展示的ECM成分影响的上皮-间充质相互作用。
