Calonje E, Fletcher C D
Department of Histopathology, St. Thomas's Hospital (UMDS), London, England.
Hematol Oncol Clin North Am. 1995 Jun;9(3):657-75.
Immunohistochemistry has a major role as an adjunct to light microscopy in the diagnosis and classification of soft tissue sarcomas. Immunohistochemical analysis of proliferation markers appears so far to add very little to accurate and experienced histologic typing of soft-tissue sarcomas. Immunohistochemical assessment of tumor suppressor genes and oncogene products gives an insight into pathogenetic mechanisms but does not appear to correlate reliably with clinical outcome. Analysis of ploidy by DNA flow cytometry does not separate benign from malignant lesions, and assessment of cell proliferation by this method does not seem to predict clinical behavior accurately.
免疫组织化学作为辅助光镜检查手段，在软组织肉瘤的诊断和分类中发挥着重要作用。迄今为止，增殖标志物的免疫组织化学分析对软组织肉瘤准确且经验丰富的组织学分型帮助甚微。肿瘤抑制基因和癌基因产物的免疫组织化学评估有助于深入了解发病机制，但似乎与临床结局并无可靠关联。通过DNA流式细胞术分析倍性无法区分良性与恶性病变，用该方法评估细胞增殖似乎也无法准确预测临床行为。
