随机扩增多态性DNA分析可快速区分儿科医院耐甲氧西林凝固酶阴性葡萄球菌血症分离株。
Randomly amplified polymorphic DNA analysis provides rapid differentiation of methicillin-resistant coagulase-negative staphylococcus bacteremia isolates in pediatric hospital.
作者信息
Bingen E, Barc M C, Brahimi N, Vilmer E, Beaufils F
机构信息
Service de Microbiologie, Hôpital Robert Debré, Paris, France.
出版信息
J Clin Microbiol. 1995 Jun;33(6):1657-9. doi: 10.1128/jcm.33.6.1657-1659.1995.
Abstract
Coagulase-negative staphylococci (CoNS) are now recognized as the most common cause of nosocomial bacteremia in pediatric patients. Randomly amplified polymorphic DNA analysis was used to study the relationships among 12 isolates of CoNS obtained from eight patients with catheter-related bacteremia in two distinct wards of our hospital and 6 epidemiologically unrelated strains. With this method, we were able to discriminate isolates that otherwise were indistinguishable by conventional criteria such as biochemical typing and antibiotic susceptibility patterns. Our results indicate that there were episodes of cross-infections among four patients in one ward but independent infectious episodes among four patients in the other ward. Randomly amplified polymorphic DNA analysis is a rapid method which seems particularly well suited to the epidemiological study of CoNS isolates.
摘要
凝固酶阴性葡萄球菌（CoNS）现已被公认为儿科患者医院获得性菌血症的最常见病因。采用随机扩增多态性DNA分析方法，对从我院两个不同病房的8例导管相关菌血症患者中分离出的12株CoNS菌株以及6株流行病学上无关联的菌株之间的关系进行了研究。通过这种方法，我们能够区分那些用生化分型和抗生素敏感性模式等传统标准无法区分的菌株。我们的结果表明，一个病房的4例患者发生了交叉感染，而另一个病房的4例患者发生的是独立感染事件。随机扩增多态性DNA分析是一种快速方法，似乎特别适合于对CoNS菌株进行流行病学研究。
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