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采用脉冲场凝胶电泳分析胎儿弯曲菌菌株。

Analysis of strains of Campylobacter fetus by pulsed-field gel electrophoresis.

作者信息

Fujita M, Fujimoto S, Morooka T, Amako K

机构信息

Department of Bacteriology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.

出版信息

J Clin Microbiol. 1995 Jun;33(6):1676-8. doi: 10.1128/jcm.33.6.1676-1678.1995.

DOI:10.1128/jcm.33.6.1676-1678.1995
PMID:7650215
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC228246/
Abstract

Campylobacter fetus chromosomal DNA from 21 strains was analyzed by pulsed-field gel electrophoresis. The fingerprint patterns generated with SmaI and SalI were distinctive. Using the profiles obtained by pulsed-field gel electrophoresis, we established the phylogenetic dendrogram of C. fetus to identify the genetic relationship of the strains.

摘要

采用脉冲场凝胶电泳分析了21株胎儿弯曲菌的染色体DNA。用SmaI和SalI酶切产生的指纹图谱具有独特性。利用脉冲场凝胶电泳获得的图谱，我们构建了胎儿弯曲菌的系统发育树状图，以确定各菌株间的遗传关系。

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本文引用的文献

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Pathogenesis of Campylobacter fetus infections: critical role of high-molecular-weight S-layer proteins in virulence.胎儿弯曲杆菌感染的发病机制：高分子量S层蛋白在毒力中的关键作用。
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Genotypic identification and phylogenetic analysis of the spotted fever group rickettsiae by pulsed-field gel electrophoresis.运用脉冲场凝胶电泳技术对斑点热群立克次体进行基因分型及系统发育分析。
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Typing of group B streptococci: comparison of pulsed-field gel electrophoresis and conventional electrophoresis.B族链球菌的分型：脉冲场凝胶电泳与传统电泳的比较
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Shift in S-layer protein expression responsible for antigenic variation in Campylobacter fetus.负责胎儿弯曲杆菌抗原变异的S层蛋白表达变化。
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Lipopolysaccharide structures of Campylobacter fetus are related to heat-stable serogroups.胎儿弯曲杆菌的脂多糖结构与热稳定血清群有关。
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Nosocomial meningitis due to Campylobacter fetus subsp. fetus in a neonatal intensive care unit.
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Use of pulsed-field agarose gel electrophoresis to size genomes of Campylobacter species and to construct a SalI map of Campylobacter jejuni UA580.使用脉冲场琼脂糖凝胶电泳对弯曲杆菌属菌种的基因组进行大小测定，并构建空肠弯曲菌UA580的SalI酶切图谱。
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