Youn B S, Jang I K, Broxmeyer H E, Cooper S, Jenkins N A, Gilbert D J, Copeland N G, Elick T A, Fraser M J, Kwon B S
Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis 46202, USA.
J Immunol. 1995 Sep 1;155(5):2661-7.
A new member of the beta-chemokine family, macrophage inflammatory protein (MIP)-related protein-2 (MRP-2) was isolated from a murine macrophage cell line, RAW 264.7. MRP-2 is composed of 122 amino acids of which the first 21 residues constitute a putative signal sequence. The putative mature protein is composed of 101 amino acids with a molecular weight of 11,600. MRP-2 is structurally similar to MIP-related protein-1 (MRP-1) (C10) and MIP-1 alpha. MRP-2 shows a 50.8% sequence identity at the protein level to MRP-1 and 46.3% identity to MIP-1 alpha. MRP-2 detects approximately 1.3 kilobase mRNA from monocyte and macrophage cell lines but does not detect the mRNA from T and B cells. The MRP-2 gene termed Scya9 was mapped to the central region of mouse chromosome 11 near the Scya1 and Scya2 genes, which are also members of the beta-chemokine superfamily. The Scya gene cluster was located between neurofibromatosis type 1 (Nf1) and myeloperoxidase (Mpo). rMRP-2 significantly suppressed colony formation by murine and human bone marrow granulocyte-macrophage (CFU-granulocyte-macrophage), erythroid (burst-forming unit-E), and multipotential (CFU-granulocyte-erythroid-macrophage-megakaryocyte) progenitor cells stimulated by combinations of growth factors.
从鼠巨噬细胞系RAW 264.7中分离出β-趋化因子家族的一个新成员，巨噬细胞炎性蛋白（MIP）相关蛋白-2（MRP-2）。MRP-2由122个氨基酸组成，其中前21个残基构成一个假定的信号序列。假定的成熟蛋白由101个氨基酸组成，分子量为11,600。MRP-2在结构上与MIP相关蛋白-1（MRP-1）（C10）和MIP-1α相似。MRP-2在蛋白质水平上与MRP-1的序列同一性为50.8%，与MIP-1α的同一性为46.3%。MRP-2可检测到单核细胞和巨噬细胞系中约1.3千碱基的mRNA，但未检测到T细胞和B细胞中的mRNA。被称为Scya9的MRP-2基因被定位到小鼠染色体11的中央区域，靠近Scya1和Scya2基因，它们也是β-趋化因子超家族的成员。Scya基因簇位于1型神经纤维瘤病（Nf1）和髓过氧化物酶（Mpo）之间。重组MRP-2显著抑制了由生长因子组合刺激的鼠和人骨髓粒细胞-巨噬细胞（CFU-粒细胞-巨噬细胞）、红系（爆式形成单位-E）和多能（CFU-粒细胞-红系-巨噬细胞-巨核细胞）祖细胞的集落形成。
