Monshipouri M, Price R R
Geo Centers Inc., Fort Washington, MD 20744, USA.
J Microencapsul. 1995 May-Jun;12(3):255-62. doi: 10.3109/02652049509010294.
A biocompatible emulsification method has been developed for microencapsulation of live cells and enzymes within a calcium alginate matrix. Fabrication of alginate beads was achieved by premixing a sequestering agent (sodium polyphosphate) and the calcium source (calcium sulphate) with the hydrogel monomer prior to the introduction to the oil phase. The competition between the sequesterant and sodium alginate in binding the available calcium ions results in a slowing down of the rate of polymerization and thereby lead to a successful calcium alginate bead formation. The mean diameter of the fabricated beads may be easily controlled by employing soy bean lecithin as an emulsifier. The polymerization time in this process may vary between 3 and 35 min depending on the ratio of sequesterant to that of calcium source at constant sodium alginate concentration. This preparation method avoids the use of pH extremes at all times and therefore is particularly suitable for encapsulating pH-sensitive cells and enzymes.
已开发出一种生物相容性乳化方法，用于在海藻酸钙基质中对活细胞和酶进行微囊化。通过在引入油相之前将螯合剂（多磷酸钠）和钙源（硫酸钙）与水凝胶单体预混合来制备海藻酸盐珠粒。螯合剂与海藻酸钠在结合可用钙离子方面的竞争导致聚合速率减慢，从而成功形成海藻酸钙珠粒。通过使用大豆卵磷脂作为乳化剂，可以轻松控制所制备珠粒的平均直径。在恒定海藻酸钠浓度下，此过程中的聚合时间可能因螯合剂与钙源的比例而异，在3至35分钟之间。这种制备方法始终避免使用极端pH值，因此特别适合封装对pH敏感的细胞和酶。
