Isoe J, Millar J G, Beehler J W
Department of Entomology, University of California, Riverside 92521, USA.
J Med Entomol. 1995 Jul;32(4):475-83. doi: 10.1093/jmedent/32.4.475.
Bioassay methods for mosquito oviposition attractants and stimulants were developed and compared, using Culex tarsalis Coq. gravid females as test insects, and distilled water, dyed water, or Bermuda grass infusions as the test stimuli. Bioassays with sticky screens covering jars containing volatile test stimuli or with colorless and odorless Triton X100 surfactant in the aqueous oviposition medium demonstrated that gravid mosquitoes were attracted to distilled water, with the attraction increased by the addition of dye, grass infusion, or the steam distillate of grass infusion to the water. A variation of the sticky screen bioassay was used to demonstrate that the grass infusion also contained oviposition stimulants. A forced oviposition bioassay was minimally useful, because it failed to discriminate between a solution known to contain oviposition stimulants and a distilled-water control. Overall, the bioassays developed can be used to distinguish between oviposition attractants and stimulants, a distinction that cannot be made by simply counting egg rafts.
开发并比较了用于蚊子产卵引诱剂和刺激物的生物测定方法，以致倦库蚊怀孕雌蚊作为测试昆虫，蒸馏水、染色水或百慕大草浸液作为测试刺激物。使用覆盖装有挥发性测试刺激物的罐子的粘性筛网进行生物测定，或在水性产卵介质中使用无色无味的吐温X100表面活性剂进行生物测定，结果表明怀孕蚊子被蒸馏水吸引，向水中添加染料、草浸液或草浸液的蒸汽馏出物会增强这种吸引力。使用粘性筛网生物测定的一种变体来证明草浸液中也含有产卵刺激物。强制产卵生物测定的作用极小，因为它无法区分已知含有产卵刺激物的溶液和蒸馏水对照。总体而言，所开发的生物测定方法可用于区分产卵引诱剂和刺激物，这一区别无法通过简单地计数卵筏来实现。
