Millar J G, Chaney J D, Mulla M S
Department of Entomology, University of California, Riverside 92521.
J Am Mosq Control Assoc. 1992 Mar;8(1):11-7.
Compounds which attract and stimulate oviposition by gravid Culex quinquefasciatus were isolated and identified from a fermented Bermuda grass infusion. The neutral portion of the ether extract of the aqueous infusion contained the stimulatory materials. Fractionation by liquid chromatography yielded an active fraction containing phenol, 4-methylphenol, 4-ethylphenol, indole and 3-methylindole. A blend of the 5 compounds strongly stimulated oviposition, as did blends of any 4 of them. Bioassays with individual compounds showed that only 3-methylindole consistently induced oviposition, in concentrations spanning 5 orders of magnitude.
从发酵的百慕大草浸出液中分离并鉴定出了能吸引并刺激致倦库蚊产卵的化合物。水浸液的乙醚提取物中的中性部分含有刺激物质。通过液相色谱进行分馏得到了一个活性馏分,其中含有苯酚、4-甲基苯酚、4-乙基苯酚、吲哚和3-甲基吲哚。这5种化合物的混合物能强烈刺激产卵,其中任意4种化合物的混合物也有同样效果。对单个化合物的生物测定表明,只有3-甲基吲哚在跨越5个数量级的浓度范围内能持续诱导产卵。