Pandya M, Pillai P, Deb M
Department of Microbiology, Lady Hardinge Medical College, New Delhi, India.
J Med Microbiol. 1995 Sep;43(3):185-8. doi: 10.1099/00222615-43-3-185.
Co-agglutination (coagg) and latex agglutination (LA) tests were used for the detection of Salmonella serotype Typhi Vi and Barber protein (BP) antigens in sera from five groups of individuals (A-E). Group A consisted of 30 blood culture-positive cases of typhoid fever and group B consisted of 30 suspected cases of typhoid fever who had sterile blood cultures but positive Widal tests. Thirty cases of pyrexia of unknown origin (PUO) were placed in group C, while group D consisted of 15 cases of septicaemia caused by organisms other than Salmonella serotype Typhi. Group E comprised 50 normal healthy individuals with no history of typhoid fever or TAB vaccination in the previous 5 years. The Vi-LA test performed best with 96.7% of group A sera and 90% of group B sera giving positive results. No false positive results and only 2.58% false negative results were obtained with this test. Considering patients with positive blood culture results or positive Widal tests as true positives, the sensitivities of the Vi-LA, BP-LA, Vi-coagg and BP-coagg tests were 93.3, 91.7, 83.3 and 86.7%, respectively. The specificities of these tests were 100, 98.5, 98.5 and 98.5%, respectively. It is suggested that the Vi-LA test can be used for the rapid and early diagnosis of typhoid fever.
采用协同凝集试验（coagg）和乳胶凝集试验（LA）检测五组个体（A - E组）血清中的伤寒沙门菌Vi血清型和巴伯蛋白（BP）抗原。A组由30例血培养阳性的伤寒热病例组成，B组由30例血培养无菌但肥达试验阳性的疑似伤寒热病例组成。30例不明原因发热（PUO）患者被归入C组，而D组由15例由非伤寒沙门菌血清型的病原体引起的败血症病例组成。E组包括50名在过去5年中无伤寒热病史或伤寒、副伤寒甲、乙三联菌苗（TAB）接种史的正常健康个体。Vi - LA试验效果最佳，A组血清中有96.7%、B组血清中有90%呈阳性结果。该试验未获得假阳性结果，仅获得2.58%的假阴性结果。将血培养结果阳性或肥达试验阳性的患者视为真阳性，Vi - LA、BP - LA、Vi - coagg和BP - coagg试验的敏感性分别为93.3%、91.7%、83.3%和86.7%。这些试验的特异性分别为100%、98.5%、98.5%和98.5%。建议Vi - LA试验可用于伤寒热的快速早期诊断。
