Jesudason M V, Sridharan G, Mukundan S, John T J
Department of Microbiology, Christian Medical College and Hospital, Vellore, India.
Diagn Microbiol Infect Dis. 1994 Feb;18(2):75-8. doi: 10.1016/0732-8893(94)90068-x.
Latex particles coated with rabbit antisera against Salmonella serotype typhi (S. typhi) Vi and O (STO) antigens were used in slide agglutination tests for the rapid identification of S. typhi in blood culture broths as soon as Gram-negative bacilli (GNB) were detected in them. Among 231 consecutive blood cultures showing GNB tested for Vi, and a subset of 163 tested for STO, by latex agglutination (LA), 125 and 32, respectively, were positive. The GNB in 127 blood cultures were confirmed by conventional methods as S. typhi, 125 (98.4%) of which had been identified by the Vi LA test. In the subset of 163, 81 grew S. typhi, of which only 32 (39.5%) had been identified by the STO LA tests. Thus, the sensitivity of the Vi and STO LA tests was 98.4% and 39.5%, respectively, whereas the specificity was 100% for both tests. Of the S. typhi isolates, 38 (30.4%) were detected by the Vi LA test on day 2 and 73 (58.4%) on day 3, day 1 being the date of inoculation of the blood culture broths. Thus, the Vi LA test is suitable for the early and rapid confirmation of S. typhi in blood culture.
用包被有兔抗伤寒沙门氏菌（S. typhi）Vi和O抗原（STO）抗血清的乳胶颗粒进行玻片凝集试验，以便在血培养肉汤中一旦检测到革兰氏阴性杆菌（GNB）时就能快速鉴定其中的伤寒沙门氏菌。在连续231份显示有GNB的血培养物中检测Vi，在163份的子集中检测STO，通过乳胶凝集试验（LA），分别有125份和32份呈阳性。127份血培养物中的GNB通过常规方法确认为伤寒沙门氏菌，其中125份（98.4%）已通过Vi LA试验鉴定出来。在163份的子集中，81份培养出伤寒沙门氏菌，其中只有32份（39.5%）通过STO LA试验鉴定出来。因此，Vi和STO LA试验的敏感性分别为98.4%和39.5%，而两种试验的特异性均为100%。在伤寒沙门氏菌分离株中，38份（30.4%）在第2天通过Vi LA试验检测到，73份（58.4%）在第3天检测到，第1天为接种血培养肉汤的日期。因此，Vi LA试验适用于血培养中伤寒沙门氏菌的早期快速确认。
