Ashby M N, Rine J
Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA.
Methods Enzymol. 1995;250:235-51. doi: 10.1016/0076-6879(95)50076-6.
We have described several quantitative and qualitative assays that have been utilized to learn the basic properties of RACE and amphibian and mammalian counterparts. Owing to powerful genetic tractability, high specific activity, and an apparently well-conserved substrate specificity, yeast is an attractive organism in which to study RACE. Efforts are currently in progress to characterize the functional role of the endoproteolytic processing step of many essential proteins.
我们已经描述了几种定量和定性分析方法，这些方法被用于了解RACE以及两栖动物和哺乳动物对应物的基本特性。由于酵母具有强大的遗传易处理性、高比活性以及明显保守的底物特异性，它是研究RACE的一种有吸引力的生物体。目前正在努力表征许多必需蛋白质的内切蛋白水解加工步骤的功能作用。
