糖基磷脂酰肌醇-磷脂酶D：一种用于糖基磷脂酰肌醇结构分析的工具。
Glycosylphosphatidylinositol-phospholipase D: a tool for glycosylphosphatidylinositol structural analysis.
作者信息
Deeg M A, Davitz M A
机构信息
Department of Medicine, University of Washington, Seattle 98195, USA.
出版信息
Methods Enzymol. 1995;250:630-40. doi: 10.1016/0076-6879(95)50101-0.
PMID:7651182
Abstract
Cleavage by the GPI-PLD provides definitive evidence of a minimal GPI structure: glucosamine-phosphatidylinositol. Unlike the case for PI-PLC, cleavage by the GPI-PLD is unaffected by acylation of the inositol ring. Thus the GPI-PLD provides an excellent simple enzymatic tool for analyzing the basic core structure of GPI anchors.
摘要
糖基磷脂酰肌醇特异性磷脂酶D（GPI-PLD）的切割提供了关于最小GPI结构（葡糖胺-磷脂酰肌醇）的确切证据。与磷脂酰肌醇特异性磷脂酶C（PI-PLC）的情况不同，GPI-PLD的切割不受肌醇环酰化的影响。因此，GPI-PLD为分析GPI锚定物的基本核心结构提供了一种出色且简单的酶学工具。
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