Desai A, Ravi V, Chandramuki A, Gourie-Devi M
Department of Neurovirology, National Institute of Mental Health and Neuro Sciences, Bangalore, India.
Microbiol Immunol. 1995;39(4):269-73. doi: 10.1111/j.1348-0421.1995.tb02200.x.
Cell-mediated immune response to Japanese encephalitis virus (JEV) and its purified envelope (E) protein was measured in 45 laboratory confirmed JE patients using a proliferation assay of peripheral blood mononuclear cells (PBMC). In parallel, JEV-specific IgM antibodies were measured by ELISA. No correlation was observed between the antibody response and results of the lymphocyte proliferation assay. Only 11 of the 42 patients positive in the antibody test were positive in the proliferation assay, and PBMC from 14/45 (31%) patients did not respond to either phytohemagglutinin and to JEV and/or its purified E protein antigen. No correlation was observed between the cell-mediated immune response and the final clinical outcome (fatality vs. recovery).
采用外周血单个核细胞（PBMC）增殖试验，对45例实验室确诊的日本脑炎（乙脑）患者进行了针对日本脑炎病毒（JEV）及其纯化包膜（E）蛋白的细胞介导免疫反应检测。同时，采用酶联免疫吸附测定法（ELISA）检测JEV特异性IgM抗体。未观察到抗体反应与淋巴细胞增殖试验结果之间存在相关性。抗体检测呈阳性的42例患者中，只有11例增殖试验呈阳性，45例患者中有14例（31%）的PBMC对植物血凝素以及JEV和/或其纯化E蛋白抗原均无反应。未观察到细胞介导免疫反应与最终临床结局（死亡与康复）之间存在相关性。
