Kamikubo T, Hayashi T, Ohkawa K
Department of Neurosurgery, Jikei University School of Medicine, Tokyo, Japan.
Neurochem Res. 1995 Apr;20(4):391-4. doi: 10.1007/BF00973092.
Ubiquitin-conjugating activities in the soluble fractions of gerbil cortex and hippocampus following transient ischemia were examined in vitro. Ten minutes of ischemia did not affect the ubiquitination of heat-denatured lysozyme both in the cortex and in the hippocampus. No reduction of the conjugating activities following ischemia was also confirmed using the partially purified ubiquitin conjugating enzymes from the cortex. These results indicate that protein ubiquitination might not be impaired following transient ischemia.
在体外检测了短暂性脑缺血后沙鼠皮层和海马体可溶部分中的泛素结合活性。十分钟的缺血对皮层和海马体中热变性溶菌酶的泛素化均无影响。使用从皮层中部分纯化的泛素结合酶，也证实了缺血后结合活性没有降低。这些结果表明，短暂性脑缺血后蛋白质泛素化可能未受损。
