Micro-homology mediated PCR targeting in Saccharomyces cerevisiae.
作者信息
Manivasakam P, Weber S C, McElver J, Schiestl R H
机构信息
Department of Molecular and Cellular Toxicology, Harvard School of Public Health, Boston, MA 02115, USA.
出版信息
Nucleic Acids Res. 1995 Jul 25;23(14):2799-800. doi: 10.1093/nar/23.14.2799.
Abstract
摘要
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本文引用的文献
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A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae.一种在酿酒酵母中进行直接基因缺失的简单高效方法。
Nucleic Acids Res. 1993 Jul 11;21(14):3329-30. doi: 10.1093/nar/21.14.3329.
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Effect of mutations in genes affecting homologous recombination on restriction enzyme-mediated and illegitimate recombination in Saccharomyces cerevisiae.影响同源重组的基因突变对酿酒酵母中限制性内切酶介导的重组和异常重组的作用。
Mol Cell Biol. 1994 Jul;14(7):4493-500. doi: 10.1128/mcb.14.7.4493-4500.1994.
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High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier.使用单链核酸作为载体对完整酵母细胞进行高效转化。
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Integration of DNA fragments by illegitimate recombination in Saccharomyces cerevisiae.酿酒酵母中通过非同源重组实现DNA片段整合
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Nucleic Acids Res. 1992 Mar 25;20(6):1425. doi: 10.1093/nar/20.6.1425.
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