用非同源DNA转化酿酒酵母：转化DNA非法整合到酵母染色体中以及转化DNA与线粒体DNA序列的体内连接。
Transformation of Saccharomyces cerevisiae with nonhomologous DNA: illegitimate integration of transforming DNA into yeast chromosomes and in vivo ligation of transforming DNA to mitochondrial DNA sequences.
作者信息
Schiestl R H, Dominska M, Petes T D
机构信息
Department of Biology, University of North Carolina, Chapel Hill 27599-3280.
出版信息
Mol Cell Biol. 1993 May;13(5):2697-705. doi: 10.1128/mcb.13.5.2697-2705.1993.
Abstract
When the yeast Saccharomyces cerevisiae was transformed with DNA that shares no homology to the genome, three classes of transformants were obtained. In the most common class, the DNA was inserted as the result of a reaction that appears to require base pairing between the target sequence and the terminal few base pairs of the transforming DNA fragment. In the second class, no such homology was detected, and the transforming DNA was integrated next to a CTT or GTT in the target; it is likely that these integration events were mediated by topoisomerase I. The final class involved the in vivo ligation of transforming DNA with nucleus-localized linear fragments of mitochondrial DNA.
摘要
当用与基因组无同源性的DNA转化酿酒酵母时，获得了三类转化体。在最常见的一类中，DNA的插入似乎是由一种反应导致的，该反应似乎需要靶序列与转化DNA片段的末端几个碱基对之间进行碱基配对。在第二类中，未检测到这种同源性，转化DNA整合到靶序列中CTT或GTT旁边；这些整合事件很可能是由拓扑异构酶I介导的。最后一类涉及转化DNA与线粒体DNA的核定位线性片段在体内的连接。
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