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Detection of heavy-ion-induced DNA double-strand breaks using static-field gel electrophoresis.

作者信息

Taucher-Scholz G, Heilmann J, Schneider M, Kraft G

机构信息

GSI Darmstadt, Germany.

出版信息

Radiat Environ Biophys. 1995 Jun;34(2):101-6. doi: 10.1007/BF01275214.

DOI:10.1007/BF01275214
PMID:7652149
Abstract

Radiation-induced DNA double-strand breaks (DSBs) were measured in Chinese hamster ovary cells (CHO-K1) using an experimental protocol involving static-field gel electrophoresis following exposure to various accelerated ions. Dose-effect curves were set up, and relative biological efficiencies (RBEs) for DSB induction were determined for different radiation qualities. RBEs around 1 were obtained for low energy deuterons (6-7 keV/microns), while for high energy oxygen ions (20 keV/microns) an RBE value slightly greater than 1 was determined. Low energetic oxygen ions (LET approximately 250 keV/microns were found to show RBEs substantially below unity, and for higher LET particles (> or = 250 keV/microns) RBEs for DSB induction were generally found to be smaller than 1. The data presented here are in line with the generally accepted view that not induced DSBs, but rather misrepaired or unrepaired DNA lesions are related to cellular inactivation.

摘要

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本文引用的文献

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Effects of different ionizing radiations on human cells in tissue culture. III. Experiments with cyclotron-accelerated alpha-particles and deuterons.不同电离辐射对组织培养中人体细胞的影响。III. 回旋加速器加速的α粒子和氘核实验。
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原子力显微镜的 DNA 研究：短 DNA 片段测量的能力。
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Lethality of heavy ion-induced DNA double-strand breaks in mammalian cells.
Int J Radiat Biol. 1993 Aug;64(2):169-78. doi: 10.1080/09553009314551261.
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