Gorelik Iu V, Kukhareva L V, Blinova M I, Pinaev G P
Tsitologiia. 1994;36(12):1205-8.
To develop a stripping model for newborn rat keratinocytes we chose appropriate culture conditions. As it has been shown for human keratinocytes, this model allows to separate the suprabasal cells from the basal cell layer, to obtain a pure population of basal-like cells. One of possible applications of this method was demonstrated in the study on the influence of extracellular matrix components on keratinocyte proliferation. A fraction of basal membrane gel, "the matrigel", was shown to decrease the uptake of 3H-thymidine by keratinocytes in the stripping model.
为了建立新生大鼠角质形成细胞的剥脱模型，我们选择了合适的培养条件。正如已在人角质形成细胞中所证实的那样，该模型能够将基底上层细胞与基底细胞层分离，从而获得纯净的基底样细胞群体。该方法的一种可能应用在关于细胞外基质成分对角质形成细胞增殖影响的研究中得到了证明。在剥脱模型中，已表明基底膜凝胶的一部分“基质胶”会降低角质形成细胞对3H-胸腺嘧啶核苷的摄取。
