Identification and sequence analysis of the E1 genomic region of a porcine adenovirus.

The complete nucleotide sequence of the putative early transcriptional region 1 (E1) of the genome of NADC-1, a porcine adenovirus, was determined. The E1 region of NADC-1 was found to be 3658 bp and located between 0 and 11.2 map units. Twelve potential open reading frames (ORFs) and five polyadenylation signals were identified in the r strand. The nucleotide sequence and each predicted amino acid sequence were compared to sequences available on a number of databases by a BLAST search and comparison. A single region of nucleotide sequence similarity was identified with the sequence of human adenovirus 5. Only 2 of the 12 potential ORFs encode polypeptides that have homology to known adenovirus polypeptides. For these predicted proteins, similarities were found with a number of adenovirus proteins. The strongest homology was found to potential E1 products of bovine adenovirus 3.