Shamardin V A, Rud' N V, Il'iasov B K
Zh Mikrobiol Epidemiol Immunobiol. 1995 Mar-Apr(2):84-6.
The effectiveness of Leptospira antigenic erythrocyte diagnosticum increases 4- to 8-fold due to the preliminary activation of erythrocytes and Boivin's antigen with 0.001-0.002 M solution of potassium periodate. ELISA with the use of conjugate based on antiglobulin serum and catalase is twice as sensitive as ELISA with conjugate on the basis of penicillinase, 3 times as sensitive as ELISA with conjugate on the basis of Leptospira hyperimmune serum and catalase and 10-20 times as sensitive as the passive hemagglutination test and the microagglutination and lysis test.
由于用0.001 - 0.002 M高碘酸钾溶液对红细胞和波伊文氏抗原进行预激活，钩端螺旋体抗原红细胞诊断液的有效性提高了4至8倍。使用基于抗球蛋白血清和过氧化氢酶的结合物的酶联免疫吸附测定（ELISA）的灵敏度是使用基于青霉素酶的结合物的ELISA的两倍，是使用基于钩端螺旋体超免疫血清和过氧化氢酶的结合物的ELISA的三倍，并且是被动血凝试验以及微量凝集和裂解试验的10至20倍。
