Takahashi M, Tomiyama S
Department of Otolaryngology, Asahikawa Medical School, Japan.
Acta Otolaryngol. 1995 May;115(3):396-9. doi: 10.3109/00016489509139336.
Cell proliferation in the endolymphatic sac (ES) after immunostimulation was assessed immunohistochemically. Guinea pigs were employed as experimental animals and keyhole limpet hemocyanin was used as the antigen. After i.p. injection of 5-bromo-2'-deoxyuridine (BrdUrd), the BrdUrd+ cells were counted to assess the number of proliferating cells. In the secondary immune reaction of the ES, the IgG+ cell count in the perisaccular region was markedly increased on days 7-14. An increased number of BrdUrd+ cells was detected, peaking on day 3, and the localization of these cells in the ES was similar to that of the IgG+ cells. This suggests that some BrdUrd+ cells may differentiate into IgG- cells within the ES.
通过免疫组织化学方法评估免疫刺激后内淋巴囊（ES）中的细胞增殖情况。使用豚鼠作为实验动物，以钥孔戚血蓝蛋白作为抗原。腹腔注射5-溴-2'-脱氧尿苷（BrdUrd）后，对BrdUrd+细胞进行计数以评估增殖细胞的数量。在ES的二次免疫反应中，囊周区域的IgG+细胞计数在第7至14天显著增加。检测到BrdUrd+细胞数量增加，在第3天达到峰值，并且这些细胞在ES中的定位与IgG+细胞相似。这表明ES内的一些BrdUrd+细胞可能分化为IgG-细胞。
