Tomiyama S, Harris J P
Division of Otolaryngology/Head and Neck Surgery, University of California, San Diego Medical Center 92103.
Acta Otolaryngol. 1989 Mar-Apr;107(3-4):202-9. doi: 10.3109/00016488909127499.
The effect of direct antigen challenge of the endolymphatic sac (e.sac) on inner ear antibody levels was investigated in guinea pigs. Two weeks following e.sac antigen inoculation, perilymph antibody levels to keyhole limpet hemocyanin (KLH) were found to be significantly elevated in the challenged ear of those animals compared to the unchallenged, opposite ear. Moreover, when the endolymphatic duct (e.duct) in a group of animals were obstructed prior to antigen challenge of their e.sac then the rise in perilymph antibody was prevented. The local accumulation of plasma cells and lymphocytes in the perisaccular space and e.sac lumen was seen following these antigen injections. These experiments suggest that inner ear antibody emanates from the e.sac and reaches the perilymphatic compartment along the route of the e.duct. Furthermore, these findings emphasize the central role of the e.sac in inner ear immunity.
研究了豚鼠内淋巴囊（e.sac）直接抗原刺激对内耳抗体水平的影响。在e.sac抗原接种两周后，发现与未受刺激的对侧耳相比，这些动物受刺激耳的外淋巴中针对钥孔血蓝蛋白（KLH）的抗体水平显著升高。此外，在一组动物的内淋巴管（e.duct）在其e.sac抗原刺激前被阻塞时，外淋巴抗体的升高被阻止。在这些抗原注射后，可见外淋巴囊周围间隙和e.sac管腔内浆细胞和淋巴细胞的局部聚集。这些实验表明，内耳抗体源自e.sac，并沿e.duct途径到达外淋巴腔。此外，这些发现强调了e.sac在内耳免疫中的核心作用。
