Henderson R J, Hill D M, Vickers A A, Edwards J M, Tillett H
J Clin Pathol. 1976 Jan;29(1):35-8. doi: 10.1136/jcp.29.1.35.
Four serological tests and three immunofluorescence tests for IgG, IgM, and IgA were compared for value in the investigation of brucellosis in veterinary surgeons. No one serological test stood out over the others, and the immunofluorescence tests did not appear to have advantages over the serological tests. If a laboratory is limited in time and resources then the saline agglutination or the complement fixation test would be reasonably satisfactory. The 2-mercaptoethanol test and the antihuman globulin (Coombs' test) have no advantages over the other two and could be dropped. Immunofluorescence tests are not recommended for routine testing of brucellosis sera. The results and these recommendations apply to the 'vet' sera tested; it is reasonable to suppose that what applies to 'vet' sera will also apply to sera of those who work with or are in repeated contact with cattle and who will have had previous experience of brucella antigen, that is, dairy farmers, herdsmen, or slaughter house employees.
对兽医布鲁氏菌病调查中用于检测IgG、IgM和IgA的四项血清学检测和三项免疫荧光检测的价值进行了比较。没有一种血清学检测比其他检测更突出，而且免疫荧光检测似乎并不比血清学检测更具优势。如果实验室在时间和资源上有限，那么盐水凝集试验或补体结合试验会相当令人满意。2-巯基乙醇试验和抗人球蛋白（库姆斯试验）并不比其他两项试验更具优势，可以舍弃。不建议将免疫荧光检测用于布鲁氏菌病血清的常规检测。这些结果和建议适用于所检测的“兽医”血清；合理推测，适用于“兽医”血清的情况也将适用于与牛接触或反复接触牛且曾接触过布鲁氏菌抗原的人员的血清，即奶农、牧民或屠宰场员工。
